10X genomics bam文件的格式

1

NA

Query name

A00682:284:HYVYMDSXX:4:1219:23954:12164

2

NA

Flag

0

3

NA

Reference name

1

4

NA

Position

12434

5

NA

Mapping quality

255

6

NA

Cigar string

130M286409N20M

7

NA

Reference name of mate

*

8

NA

Position of mate

0

9

NA

Template length

0

10

NA

Sequence

CTCCAAAGA....ATCAAAAAAAAAAAAAA

11

NA

Sequence quality

FFFFFFFFFFFFFFFFFF....

12

NH

Number of reported alignments for query

NH:i:1

13

HI

Query hit index

HI:i:1

14

AS

Alignment score

AS:i:135

15

nM

Number of mismatches per pair

nM:i:5

16

RE

Region type (E = exonic, N = intronic, I = intergenic)

RE:A:I

17

BC

Sample index read

BC:Z:TCGCCAGC

18

QT

Sample index read quality

QT:Z:FFFFFFFF

19

CR

Cell barcode

CR:Z:GCGTGCACATAGAAAC

20

CY

Cell barcode read quality

CY:Z:FFFF:FFFFFF,FFFF

21

CB

Cell barcode that is error-corrected and confirmed against a list of known-good barcode sequences

CB:Z:GCGTGCACATAGAAAC-1

22

UR

Unique Molecular Identifier (UMI)

UR:Z:CGCTCACGCATA

23

UY

UMI read quality

UY:Z::FFFFFFFFFFF

24

UB

UMI that is error-corrected among other molecular barcodes with the same cellular barcode and gene alignment

UB:Z:CGCTCACGCATA

25

RG

Read group

RG:Z:Day1:0:1:HWJ5VDSXX:4